rna (r6625, torula yeast, type vi)  (Merck KGaA)

Journal: Frontiers in Molecular Neuroscience

Article Title: Loss-of-Function Models of the Metabotropic Glutamate Receptor Genes Grm8a and Grm8b Display Distinct Behavioral Phenotypes in Zebrafish Larvae ( Danio rerio )

doi: 10.3389/fnmol.2022.901309

Figure Lengend Snippet: Pattern of grm8a and grm8b expression in the developing zebrafish revealed by whole-mount RNA in situ hybridization. (A) Grm8a expression at 24, 36, and 48 hpf in the developing zebrafish. Lateral (top) and dorsal (bottom) overviews indicate expression as early as 24 hpf in the central nervous system. (B) Grm8b expression at 24, 36, and 48 hpf in the developing zebrafish. Lateral (top) and dorsal (bottom) overviews indicate prominent expression as early as 24 hpf in the central nervous system. Details on the individual expression patterns are described in the main text. Arrowheads indicate transcript labeling in individual cell cluster of the medulla oblongata (MO). All images are oriented with anterior to the left. Abbreviations are listed in . Scale bar is 100 μm.

Article Snippet: The specimens were then post-fixed in 4% PFA, washed in PBST and were incubated in hybridization buffer containing 5 mg/ml torula yeast RNA type VI (Merck KGaA, Darmstadt, Germany) for 1 h at 65°C.

Techniques: Expressing, RNA In Situ Hybridization, Labeling

Journal: Frontiers in Molecular Neuroscience

Article Title: Loss-of-Function Models of the Metabotropic Glutamate Receptor Genes Grm8a and Grm8b Display Distinct Behavioral Phenotypes in Zebrafish Larvae ( Danio rerio )

doi: 10.3389/fnmol.2022.901309

Figure Lengend Snippet: Grm8a expression pattern in adult zebrafish brain labeled by RNA in situ hybridization. (A–M) Grm8a transcript labeling by RNA in situ hybridization on cross-sections of an adult zebrafish brain. Images are arranged from anterior to posterior as indicated by the scheme at the top. Details on grm8a expression pattern are described in the main text. Anatomical abbreviations are listed in . Scale bar is 200 μm.

Article Snippet: The specimens were then post-fixed in 4% PFA, washed in PBST and were incubated in hybridization buffer containing 5 mg/ml torula yeast RNA type VI (Merck KGaA, Darmstadt, Germany) for 1 h at 65°C.

Techniques: Expressing, Labeling, RNA In Situ Hybridization

Journal: Frontiers in Molecular Neuroscience

Article Title: Loss-of-Function Models of the Metabotropic Glutamate Receptor Genes Grm8a and Grm8b Display Distinct Behavioral Phenotypes in Zebrafish Larvae ( Danio rerio )

doi: 10.3389/fnmol.2022.901309

Figure Lengend Snippet: Grm8b expression pattern in adult zebrafish brain labeled by RNA in situ hybridization. (A–L) Grm8b transcript labeling by RNA in situ hybridization on cross-sections of an adult zebrafish brain. Images are arranged from anterior to posterior as indicated by the scheme at the top. Details on grm8b expression pattern are described in the main text. Anatomical abbreviations are listed in . Scale bar is 200 μm.

Article Snippet: The specimens were then post-fixed in 4% PFA, washed in PBST and were incubated in hybridization buffer containing 5 mg/ml torula yeast RNA type VI (Merck KGaA, Darmstadt, Germany) for 1 h at 65°C.

Techniques: Expressing, Labeling, RNA In Situ Hybridization

Journal: Frontiers in Molecular Neuroscience

Article Title: Loss-of-Function Models of the Metabotropic Glutamate Receptor Genes Grm8a and Grm8b Display Distinct Behavioral Phenotypes in Zebrafish Larvae ( Danio rerio )

doi: 10.3389/fnmol.2022.901309

Figure Lengend Snippet: Grm8a is partly expressed in gad1a positive neurons in the developing zebrafish brain. (A) Whole-mount RNA in situ hybridization of grm8a expression at 36 hpf. (B) Magnified picture of the region boxed in (A) . Scale bar, 200 μm (overview) and 100 μm (magnification). (C–T) Double labeling of grm8a (blue) and gad1a (red) expression in 36 hpf old embryos using two-color RNA in situ hybridization. Lateral (C,D) and dorsal (F,G) overview of embryonic CNS with anterior to the left. (E,H) Magnifications of boxed areas in (C,G) . Dashed lines in (C,D) indicate cutting sites for cross sections shown in (I–R) . (M–P,S,T) Magnifications of boxed areas in (I–L,Q,R) , respectively. Arrowheads indicate sites of co-localization. Abbreviations are listed in . Scale bars: 100 μm (overview), 50 μm (magnified images).

Article Snippet: The specimens were then post-fixed in 4% PFA, washed in PBST and were incubated in hybridization buffer containing 5 mg/ml torula yeast RNA type VI (Merck KGaA, Darmstadt, Germany) for 1 h at 65°C.

Techniques: RNA In Situ Hybridization, Expressing, Labeling